Culture-adapted Plasmodium falciparum isolates from UK travellers: in vitro drug sensitivity, clonality and drug resistance markers

Genetic alteration in drug resistance markers of Plasmodium falciparum.

Plasmodium falciparum shows plasticity in its genome. For its survival it can delete certain genes (or portions) if not needed for its growth and has the capability to regulate its genes under various stages of its life cycle as well as under unfavourable environmental conditions. Parasite shows enormous amount of antigenic variation under immune pressure leading to the emergence of vaccine res...

In vitro tests for drug resistance in Plasmodium falciparum

Assessment of Markers of Antimalarial Drug Resistance in Plasmodium falciparum Isolates from Pregnant Women in Lagos, Nigeria

BACKGROUND The use of antimalarial drugs for prevention and treatment is a major strategy in the prevention of malaria in pregnancy. Although sulphadoxine-pyrimethamine (SP) is currently recommended for intermittent preventive treatment of malaria during pregnancy in Nigeria, previously used drugs for prophylaxis such as chloroquine (CQ) and pyrimethamine are accessible as they are purchased ov...

In Vitro Antimalarial Drug Sensitivity Testing For Plasmodium falciparum and Plasmodium vivax

Aim of this study was to standardize the Antimalarial drug sensitivity testing using microtitre plate method similar to WHO III plate method. The study was conducted over a period of two years from January 2013 to December 2014. Total 44 patient samples were included in this study (22 were P. falciparum and 22 were P. vivax). 68.18% of P. falciparum blood samples showed resistance to chloroquin...

In vitro antimalarial activity of tigecycline against Plasmodium falciparum culture-adapted reference strains and clinical isolates from the Brazilian Amazon.

INTRODUCTION We evaluated the in vitro antimalarial activity of tigecycline as an alternative drug for the treatment of severe malaria. METHODS A chloroquine-sensitive Plasmodium falciparum reference strain, a chloroquine-resistant reference strain, and three clinical isolates were tested for in vitro susceptibility to tigecycline. A histidine-rich protein in vitro assay was used to evaluate ...